Genetic models

Mouse overexpressing neuropeptide Y under the promoter of dopamine-beta-hydroxylase in adrenergic and noradrenergic neurons presents with a Metabolic Syndrome -like phenotype with increased fat mass and impaired glucose tolerance without hyperphagia. The mice are susceptible to induction of vascular hypertrophy. Ruohonen et al Transgenic mice overexpressing neuropeptide Y in noradrenergic neurons: A novel model of increased adiposity and impaired glucose tolerance. Diabetes, 57(6):1517-25, 2008. Ruohonen et al Sympathetic nervous system-targeted neuropeptide Y overexpression in mice enhances neointimal formation in response to vascular injury. Peptides 30:715-720, 2009.

Contact
Eriika Savontaus, MD, PhD

Email: eriika.savontaus(at)utu.fi

 
Metabolic syndrome
 

Diet-induced model

Diets high in fat and/or sucrose content are used to induce fat mass gain, insulin resistance, hyperlipidemia, hypercholesterolemia, hepatosteatosis and impaired vascular function. Heinonen I et al The effects of equal caloric high fat and western diet on metabolic syndrome, oxidative stress and vascular endothelial function in mice. Acta Physiologica, 211: 515–527 Rinne P et al α-Melanocyte-stimulating hormone regulates vascular NO availability and protects against endothelial dysfunction. Cardiovasc Res. 97:360-368, 2013

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Eriika Savontaus, MD, PhD

Email: eriika.savontaus(at)utu.fi

 
Diet induced obesity
 

Type 1 Diabetes

Non-obese diabetic (NOD) mice develop an autoimmune diabetes spontaneously with age. The model can be used to study pathogenesis and prevention of type 1 diabetes.Sysi-Aho M et al Metabolic regulation in progression to type 1 diabetes, PLoS Computational Biology 7:e1002257, 2011. Alam CM et al. Keratin 8 modulates ß-cell stress responses and normoglyceamia. J. Cell Sci. 126:5635-5644, 2013.

Contact
Eriika Savontaus, MD, PhD
Email: eriika.savontaus(at)utu.fi

Diana Toivola, PhD
Email: dtoivola(at)abo.fi

 
Autoimmune diabetes
 

Chemically induced model of diabetes

Streptozotocin (STZ)  is used to destroy pancreatic β-cells, reduce the production of insulin leading to hyperglycemia and a commonly used model for of Type I diabetes. Acute and chronic diabetic phenotypes are studied. Combining low-dose STZ with diet-induced insulin release is used to model type 2 diabetes.Alam CM et al. Keratin 8 modulates ß-cell stress responses and normoglyceamia. J. Cell Sci. 126:5635-5644, 2013.

Contact
Diana Toivola, PhD
Email: dtoivola(at)abo.fi

Eriika Savontaus, MD, PhD
Email: eriika.savontaus(at)utu.fi

 
Type 1 and type 2 diabetes