The Isolated Perfused Heart System, as originated by Oscar Langendorff at late 19th century, is a predominant technique in pharmacological and physiological research.Radnoti Isolated Working Heart System may be used both in Langendorff and Working Heart modes. In Langendorff method, aorta (left ventricle) is cannulated and perfused with Krebbs- Henseleit solution. A constant hydrostatic pressure is used. A left ventricular pressure measurement is performed to evaluate the effects of drugs used on ventricular function e.g. pressure and heart rate. In Working Heart mode, the heart is perfused through the left atria. Perfusate enters the left ventricle and is ejected out of the aorta, mimicking normal heart function. Both preload (left atria) an afterload (left ventricle) pressures and heart rate may be recorded with ECG and heart temperature measurements. Both constant pressure and constant flow models for isolated heart as well as both recirculating and non-recirculating modes are provided. The system allows to screen new drug candidates.

Contacts
Petteri Rinne, PhD

Email: petteri.rinne(at)utu.fi


Tomi Streng, PhD
Adjunct Professor of Animal Physiology  

Email: tomi.streng(at)utu.fi

Isolated heart