Analysis of intestinal phenotypes of mouse intestine and related physiological parameters

In vivo analysis

  • Colitis disease activity index from mouse weight, stool hydration and consistency
  • In vivo imaging of inflammation (ROS/NOS) in live mice for longitudinal analysis
  • Blood sampling

Post-mortem analysis

  • Assessment of tumor number and size
  • Assessment of inflammation by myeloperoxidase (MPO) staining of cryosections
  • Morphological analysis of intestinal tissues
  • Analysis of intestinal cell types, proliferation and migration
  • Histological scoring of colitis
  • Crypt length
  • Number and location of epithelial cell types
  • Enterocyte proliferation and migration

Intestinal cell isolation and culture

  • Isolation of colonic and small intestinal epithelial crypts and villi
  • Crude isolation of intestinal epithelial by scrapings of mucosa

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Contact
Diana Toivola, PhD

Email: dtoivola(at)abo.fi

Intestinal Analysis Methods
Phenotype the intestine in your model!