Transgenic mice are generated by injecting cloned DNA into zygotes which are then transferred in pseudopregnant females to develop to term. Mouse strains routinely used as zygote donors are FVB/N and C57Bl/6N. Genotyping samples are taken for screening potential founders by PCR or southern blot. A robust screening method must be established before submitting DNA for microinjection. Service includes purification of the DNA construct, microinjection, transfer of 300 embryos and taking genotyping samples.

Contact
Petra Sipilä, PhD
Adjunct Professor of Reproductive and Developmental Biology

Email: petra.sipila(at)utu.fi

Transgenic Mouse Production
Traditional and BAC transgenesis