Urodynamical recordings, describing functional aspects of the lower urinary tract (LUT), are provided. Anaesthetized rodents are used to study LUT function.  Micturition is evoked by saline (0.9% NaCl) infusion to urinary bladder via a tubing, which is also used for transvesical cystometry (bladder pressure) recordings. The cannula is connected to a microinfusion pump and to a pressure transducer connected to an amplifier. An ultrasonic flow probe placed around the most distal part of the urethra and connected to a flow meter (Transonic Systems) is used for measurement of the “urine” flow rate. At the same time as the measurements of transvesical cystometry and flow rate, the electrical activity of the rhabdosphincter muscle is recorded extracellularly by a monopolar suction electrode, attached onto the ventral surface of the muscle.Continuous recordings are obtained by using Acq Knowledge 3.5.3 software (Biopac Systems). Also awake rodents may be used in urodynamical recearch.

Tomi Streng, PhD
Adjunct Professor of Animal Physiology

Email: tomi.streng(at)utu.fi