| Transgenic mice are generated by injecting cloned DNA into zygotes which are then transferred in pseudopregnant females to develop to term. Mouse strains routinely used as zygote donors are FVB/N and C57Bl/6N. Genotyping samples are taken for screening potential founders by PCR or southern blot. A robust screening method must be established before submitting DNA for microinjection. Service includes purification of the DNA construct, microinjection, transfer of 300 embryos and taking genotyping samples. |
Contact Email: petra.sipila(at)utu.fi |
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| Traditional and BAC transgenesis | |