Using pronuclear injection TCDM has generated more than 1100 transgenic founder mice from over 170 transgenes. Furthermore, 58 gene targetings has been carried out with germ line transmission. Examples of research articles recently published with GM mouse models generated by TCDM are shown below.

2017

Latonen L, Scaravilli M, Gillen A, Hartikainen S, Zhang FP, Ruusuvuori P, Kujala P, Poutanen M, Visakorpi T. In Vivo Expression of miR-32 Induces Proliferation in Prostate Epithelium. Am J Pathol. 2017 Aug 19. pii: S0002-9440(17)30049-4. doi: 10.1016/j.ajpath.2017.07.012.

Heinonen J, Zhang FP, Surmann-Schmitt C, Honkala S, Stock M, Poutanen M, Säämänen AM. Defects in chondrocyte maturation and secondary ossification in mouse knee joint epiphyses due to Snorc deficiency. Osteoarthritis Cartilage. 2017 Jul;25(7):1132-1142. doi: 10.1016/j.joca.2017.03.010.

Da Ros M, Lehtiniemi T, Olotu O, Fischer D, Zhang FP, Vihinen H, Jokitalo E, Sironen A, Toppari J, Kotaja N. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy. 2017 Feb;13(2):302-321.

Lehti MS, Zhang FP, Kotaja N, Sironen A. SPEF2 functions in microtubule-mediated transport in elongating spermatids to ensure proper male germ cell differentiation. Development. 2017 Jul 15;144(14):2683-2693.

2016

Tammimäki A, Aonurm-Helm A, Zhang FP, Poutanen M, Duran-Torres G, Garcia-Horsman A, Männisto PT. Generation of membrane-bound catechol-O-methyl transferase deficient mice with disctinct sex dependent behavioral phenotype. J Physiol Pharmacol. 2016 Dec;67(6):827-842.

Koli K, Sutinen E, Rönty M, Rantakari P, Fortino V, Pulkkinen V, Greco D, Sipilä P and Myllärniemi M. Gremlin-1 overexpression in mouse lung reduces silica-induced lymphocyte recruitment – a link to idiopathic pulmonary fibrosis through negative correlation with CXCL10 chemokine. PLoS One 2016 Jul 18;11(7):e0159010. doi: 10.1371/journal.pone.0159010.

Morton NM, Beltram J, Carter RN, Michailidou Z, Gorjanc G, McFadden C, Barrios-Llerena ME, Rodriguez-Cuenca S, Gibbins MT, Aird RE, Moreno-Navarrete JM, Munger SC, Svenson KL, Gastaldello A, Ramage L, Naredo G, Zeyda M, Wang ZV, Howie AF, Saari A, Sipilä P, Stulnig TM, Gudnasson V, Kenyon CJ, Seckl JR, Walker BR, Webster SP,  Dunbar DR, Churchill GA, Vidal-Puig A, Fernandez-Real JM, Emilsson V, Horvat S. Genetic identification of thiosulfate sulfurtransferase as an adipocyte-expressed antidiabetic target in mice selected for leanness. Nat Med. 2016 Jun 6. doi: 10.1038/nm.4115.

Calo N, Ramadori P, Sobolewski C, Romero Y, Maeder C, Fournier M, Rantakari P, Zhang FP, Poutanen M, Dufour JF, Humar B, Nef S, Foti M. Stress-activated miR-21/miR-21* in hepatocytes promotes lipid and glucose metabolic disorders associated with high-fat diet consumption. Gut  doi: 10.1136/gutjnl-2015-310822. [Epub ahead of print]

Björkgren I, Alvarez L, Blank N, Balbach M, Turunen H, Laajala TD, Toivanen J, Krutskikh A, Wahlberg N, Huhtaniemi I, Poutanen M, Wachten D, Sipilä P. Targeted inactivation of the mouse beta-defensin41 alters sperm flagellar beat pattern and zona pellucida binding.  Mol Cell Endocrinol  427:143-54, 2016.

Määttä JA, Bendre A, Laanti M, Büki KG, Rantakari P, Tervola P, Saarimäki J, Poutanen M, Härkönen P, Väänänen K. Fam3c modulates osteogenic cell differentiation and affects bone volume and cortical bone mineral density. BoneKey Reports, 5:787, 2016.

2015

Sareila O, Hagert C, Rantakari P, Poutanen M, Holmdahl R. Direct Comparison of a Natural Loss-of-Function Single Nucleotide Polymorphism with a Targeted Deletion in the Ncf1 Gene Reveals Different Phenotypes. PLoS One 10(11):e0141974. doi: 10.1371/journal.pone.0141974, 2015

Modi H, Jacovetti C, Tarussio D,  Metref S, Madsen O, Zhang FP, Rantakari P, Poutanen M, Nef S, Gorman T, Regazzi R, Thorens B. Autocrine action of  IGF2 regulates adult β cell mass and function. Diabetes 64(12):4148-57, 2015.

Hakkarainen J, Jokela H, Pakarinen P, Heikelä H, Kätkänaho L, Vandenput L, Ohlsson C, Zhang FP, Poutanen M. Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production. FASEB J 29:3806-16, 2015

2014

Movérare-Skrtic S, Henning P, Liu X, Nagano K, Saito H, Börjesson AE, Sjögren K, Windahl SH, Farman H, Kindlund B, Engdahl C, Koskela A, Zhang FP, Eriksson EE, Zaman F, Hammarstedt A, Isaksson H, Bally M, Kassem A, Lindholm C, Sandberg O, Aspenberg P, Sävendahl L, Feng JQ, Tuckermann J, Tuukkanen J, Poutanen M, Baron R, Lerner UH, Gori F, Ohlsson C. Osteoblast-derived WNT16 represses osteoclastogenesis and prevents cortical bone fragility fractures. Nat Med 20:1279-88, 2014.

Karikoski M, Marttila-Ichihara F, Elima K, Rantakari P, Hollmén M, Kelkka T, Gerke H, Huovinen V, Irjala H, Holmdahl R, Salmi M, Jalkanen S. Clever-1/stabilin-1 controls cancer growth and metastasis. Clin Cancer Res 20:6452-64, 2014.

2013

Strauss L, Rantakari P, Sjögren K, Salminen A, Lauren E, Kallio J, Damdimopoulou P, Boström M, Boström PJ, Pakarinen P, Zhang F, Kujala P, Ohlsson C, Mäkelä S, Poutanen M. Seminal vesicles and urinary bladder as sites of aromatization of androgens in men, evidenced by a CYP19A1-driven luciferase reporter mouse and human tissue specimens. FASEB J 2013 27:1342-50.

2012

Ventelä S, Come C,  Mäkelä J-A, Hobbs RM, Mannermaa L, Kallajoki M, Chan EK, Pandolfi PP, Toppari J and Westermarck J. CIP2A promotes proliferation of spermatogonial progenitor cells and spermatogenesis in mice. PLoS One 2012 7(3):e33209. doi: 10.1371/journal.pone.0033209.

Turunen HT, Sipilä P, Krutskikh A, Toivanen J, Mankonen H, Hämäläinen V, Björkgren I, Huhtaniemi I, Poutanen M. Loss of cysteine-rich secretory protein 4 (Crisp4) leads to deficiency in sperm-zona pellucida interaction in mice. Biol Reprod 2012 16;86(1):1-8.

Turunen HT, Sipilä P, Strauss L, Björkgren I, Huhtaniemi I, Poutanen M. Loss of Bmyc results in increased apoptosis associated with upregulation of Myc expression in juvenile murine testis. Reproduction 2012 144(4):495-503.

Krutskikh A, Poliandri A, Cabrera-Sharp V, Dacheux JL, Poutanen M, Huhtaniemi I. Epididymal protein Rnase10 is required for post-testicular sperm maturation and male fertility. FASEB J. 2012 26(10):4198-209.

2010

Rantakari P, Nikkilä J, Jokela H, Ola R, Pylkäs K, Lagerbohm H, Sainio K, Poutanen M, Winqvist R. Inactivation of Palb2 gene leads to mesoderm differentiation defect and early embryonic lethality in mice. Hum Mol Genet. 2010 19(15):3021-9.

Jokela H, Rantakari P, Lamminen T, Strauss L, Ola R, Mutka AL, Gylling H, Miettinen T, Pakarinen P, Sainio K, Poutanen M. Hydroxysteroid (17beta) dehydrogenase 7 activity is essential for fetal de novo cholesterol synthesis and for neuroectodermal survival and cardiovascular differentiation in early mouse embryos. Endocrinology 2010 151(4):1884-92.

Rantakari P, Lagerbohm H, Kaimainen M, Suomela JP, Strauss L, Sainio K, Pakarinen P, Poutanen M. Hydroxysteroid (17{beta}) dehydrogenase 12 is essential for mouse organogenesis and embryonic survival. Endocrinology 2010 151(4):1893-901.

Rantakari P, Strauss L, Kiviranta R, Lagerbohm H, Paviala J, Holopainen I, Vainio S, Pakarinen P, Poutanen M. Placenta defects and embryonic lethality resulting from disruption of mouse hydroxysteroid (17-beta) dehydrogenase 2 gene. Mol Endocrinol 2008 22(3):665-75.