Spheroids
Spheroid cultures involve the 3D growth of cell lines within hydrogels. Spheroids can originate from single cells or cell clusters and may exhibit invasive growth. The biophysical and biochemical properties of these 3D cultures are designed to mimic the in vivo environment, thereby enhancing the physiological relevance of the obtained data. TCDM provides support in experimental planning and, in some cases, execution.
Primary cell organoids
Organoids are self-organizing 3D clusters of cells derived from stem cells or tumor cells in vitro, retaining key structural and functional characteristics of their tissue of origin. They offer a physiologically relevant model for studying developmental processes, tumor biology, and drug responses. TCDM provides expertise in designing experiments involving organoid cultures from mouse or human primary cells. The customer is responsible for supplying the primary cells for these studies.
Tissue explant cultures
Tissue explants are fragments of fresh tissue cultured ex vivo, partially preserving the original architecture, cellular heterogeneity, and microenvironment. They are commonly used in human and mouse tumor studies, providing a more accurate representation of tumor behavior in the presence of tumor-associated stromal cells and extracellular matrix. TCDM provides expertise in planning and, in some cases, executing these experiments. The customer is responsible for supplying the tissue material for the studies.
