Production of patient specific cell lines
Somatic cells can be reprogrammed to the pluripotent stage. Produced hPSC lines can be differentiated into various cell types to study patient-specific phenotypes. At the moment, we use a non-integrative Sendai virus to reprogram somatic cells (peripheral blood mononuclear cells or skin fibroblasts). Please be aware that ethical approval from the local ethics committee is necessary before starting the derivation of hiPSCs. TCDM provides expertise in planning or executing these experiments.
Validation of Pluripotency
Once a new human-induced pluripotent stem cell line is generated, it must be characterized. This includes validation of normal morphology, immunofluorescence staining of undifferentiated hPSC markers, quantitative flow cytometric analysis of the presence of undifferentiated/differentiated cells, generation of the embryonic bodies, and expression of three germ layers (endo-, ecto-, and mesoderm) in them measured by RT-PCR/immunofluorescence, karyotype based on G-banding, Sanger sequencing of possible patient-specific mutations, mycoplasma test and absence of Sendai virus (RT-PCR of SeV, c-Myc, Klf-4, and KOS transgenes in early-infected cells and established cell lines). TCDM provides expertise in planning or executing these experiments.
Expansion of human pluripotent stem cells
There is no need to continuously create new cell lines tailored to each patient. Hundreds of pluripotent stem cell lines ready to be used can be found in public databases, such as the human pluripotent stem cell registry: https://hpscreg.eu/. Conservative estimates suggest that over 20,000 human pluripotent stem cells (hPSCs), including iPSC and embryonic stem cells (ESCs), have been generated worldwide, including various disease-specific cell lines (Hu et al. Stem Cell Reports. 2025 Oct 14;20(10):102644). TCDM provides expertise in culturing hPSC lines in various culture conditions. We can also help to amplify a stock library from the purchased vial. In addition, we can provide locally generated hIPSC lines for your research: UTUi001-A (TUR1), UTUi001-B (TUR2), UTUi001-C (TUR3), and UTUi003-A (TUR-B1).