Analysis of intestinal phenotypes of mouse intestine and related physiological parameters
In vivo analysis
- Colitis disease activity index from mouse weight, stool hydration and consistency
- In vivo imaging of inflammation (ROS/NOS) in live mice for longitudinal analysis
- Blood sampling
Post-mortem analysis
- Assessment of tumor number and size
- Assessment of inflammation by myeloperoxidase (MPO) staining of cryosections
- Morphological analysis of intestinal tissues
- Analysis of intestinal cell types, proliferation and migration
- Histological scoring of colitis
- Crypt length
- Number and location of epithelial cell types
- Enterocyte proliferation and migration
Intestinal cell isolation and culture
- Isolation of colonic and small intestinal epithelial crypts and villi
- Crude isolation of intestinal epithelial by scrapings of mucosa
- 3D organoid (mini-gut) culture
Intestine-specific inducible Cre-lines for floxed gene analysis
- Villin-Cre-ERT2 Tamoxifen-induced mice
- Villin-Cre1000 mice
- CDX2-CreERT2 Tamoxifen- induced mice
