In addition to the facilities for mouse embryonic stem cell culture TCDM hosts three cell culture facilities for culturing cancer cell lines and primary cells. We utilize routinely several cancer cell lines, and have produced novel cell lines by immoralization in vivo in transgenic mice (Sipilä et al., Endocrinology, 145(1):437-46, 2004). Fluorescent cell lines are produced by lenti-viral infections by collaboration, and several cell lines expressing fluorescent proteins for in vivo imaging has been produced. Furthermore, patient derived cell lines have been produced.
Patient derived cells
As regards the generation of patient-derived cell lines, we will employ a method where the tissue obtained from the patient or mice is first propagated in cell culture by using feeder cells and Rho kinase inhibitor (Liu et al., Am J Pathol. 180:599-607, 2012). This novel technique is expected to allow efficient expansion of epithelial cells for in vitro experiments, xenografting and genetic manipulation. This method is currently being established in our laboratory in collaboration with Dr. Chris Albanese (Departments of Pathology and Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington DC, USA). Currently human cell lines are generated from normal and malignant prostate epithelium, endometrium and endometriotic lesions.
Taija Heinosalo, PhD
|Patient derived cell lines for personalized medicine.|