Culturing and manipulating of mouse embryonic stem cells (mES cells) in pluripotent stage require fastidious techniques and specialized reagents. TCDM, thus, provides service and collaboration in culturing high quality ES cells and their targeting. The mES cell lines typically used for targeting in TCDM are G4 129S6/C57Bl/6 hybrid cells and JM8.N4 C57Bl/6 cells. However, other cell lines are also available.
- Culture of mES cells
- Targeting of knock-out and knock-in constructs into mES cells including picking and screening of 400-600 clones
- Selection marker removal from targeted mES cells with either FLP or Cre recombinase
- Preparation of single cell suspension for blastocyst injection
- Mycoplasma testing